Tritium Synthesis

Project Overview

ViTrax has extensive experience in preparing a wide range of tritiated products. We regularly handle a variety of compounds including, but not limited to, typical small molecule drugs, peptides, proteins, polymers, nucleosides, nucleotides, and oligonucleotides.

Our project scope ranges from single-step, straight forward synthesis to multistep, sensitive macromolecules. We are accustomed to handling minute quantities of precious materials and understand their value to our customers.

ViTrax respects the confidentiality commonly associated with custom contract synthesis. We welcome written confidentiality agreements and can assist by providing a standard boiler plate agreement if you so choose.

Tritium Incorporation

The following are the more common techniques available for introducing tritium into your target molecule:

  • Catalytic Reduction with Tritium Gas
  • Reduction with Sodium Borohydride, [3H]
  • Reduction with Lithium Aluminum Hydride, [3H]
  • Catalytic exchange with Tritium Gas
  • Anion quench with Tritiated Water
  • Alkylation with Methyl Iodide, [3H]

Product Purification

Most tritiated intermediates and final products are typically purified by Semi-Prep or preparative HPLC methods. TLC, Paper Chromatography, low pressure columns and other wet techniques are also applied in custom cases.

Product Analysis

Final product analysis requiring more than the typical HPLC radiotrace should be discussed during the quote request process. ViTrax can provide a full analytical package including any of the following applications:

  • HPLC Radioflow analysis
  • HPLC with monochromatic UV or PDA analysis
  • TLC Radioscan
  • T-NMR


As the stability of a product is dependent upon its storage method and rate of use, the manner in which it is packaged is an important consideration. Packaging solvent and the number of units required should be discussed during the quote request process.


All final products are shipped overnight on dry or wet ice.


Costs are assessed based upon all of the above items and can vary widely. ViTrax offers technical support to help achieve the most cost effective and technically sound approach to meet your needs. Call for further information.